BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI. |
Specifications
Application | Molecular Cloning Restriction site mapping Genotyping SNP |
Concentration | 10U/μL |
Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µL. |
Source | E. coli |
Shipping, Reconstitution, & Storage
Form | Liquid |
Stability And Storage | Store at -20°C ± 5°C for up to 24 months. Avoid repeated freeze/thaw cycles. Transport on dry ice. |
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