BsaI, GMP grade

  • Description:
  • Recognizes asymmetric DNA sequences and cleaves outside of their recognition sequence
  • Applications: 
  • Genotyping, SNP, Plasmid linearization
  • Product Features:
  • Special formulation enables BSA-free reaction
    Manufactured according to GMP guidelines
    Antibiotic-free and animal-free production

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BsaI is designed to be used upstream of the in vitro mRNA synthesis step during mRNA therapeutics and vaccine manufacturing. As one of the type IIS restriction enzymes, it recognizes asymmetric DNA sequences and cleaves outside of their recognition sequence.
The cleavage site of BsaI is GGTCTC (N1/N5), where the GGTCTC acts as the recognition site, and the N1/N5 represents the cleavage site with a 5′ overhang. We provides a BSA-free reaction buffer to ensure the safety of mRNA vaccine production

Specifications
Application Genotyping
SNP
Plasmid linearization
Concentration 20U/µL
Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µL.
Source Expressed in an E.coli strain that carries the BsaI gene from Bacillus stearothermophilus.
Quality Standards Activity (Digestion activity detection): ≥ 20 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual DNase: Negative
Residual RNase: Negative
Residual Host Protein: ≤ 20 ng/mg
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Heavy Metal: ≤ 10 ppm
Bioburden: ≤ 1 CFU/10mL
Shipping, Reconstitution, & Storage
Form Liquid
Shipping Shipping with blue ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Packages

10 KU, 100 KU, 20 KU

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